SARS-CoV-2 Nucleoprotein (NP) (85B4) rabbit mAb
We have leveraged our next-generation rabbit mAb discovery platform to develop recombinant rabbit monoclonal antibodies with extremely high sensitivity and specificity to SARS-CoV-2. These antibodies, which include clones 75G5a (Abwiz Cat. #2481), 84C4a (Abwiz Cat. #2486), 84D7 (Abwiz Cat. #2491), 85C1 (Abwiz Cat. #2496), 85C10 (Abwiz Cat. #2506), 85B4 (Abwiz Cat. #2511), and 85E9 (Abwiz Cat. #2516) can be paired in sandwich detection assay and used to detect nucleoprotein (NP) antigen from SARS-CoV-2. Antibodies 75G5a, 84C4a, 84D7, 85C10, and 85B4 do not cross-react to the highly related SARS-CoV virus or to any other coronaviruses tested. Sandwich ELISA detection using TMB/acid developer reliably detects NP antigen in the pg/mL range, and sensitivity is expected to be even higher when using more sensitive developer strategies. This panel of antibodies can be used as raw materials for diagnostic kits and can be applied to lateral flow systems for diagnostic detection of COVID-19.
| Applications | ELISA |
|---|---|
| Clone | CoV2NP-85B4 |
| Format | Unconjugated |
| Validated Reactivity | Other |
| Cross Reactivity | Highly-specific to SARS-CoV-2 nucleoprotein; does not cross-react with nucleoprotein from SARS-CoV or other coronaviruses. |
| Detection | Anti-Rabbit IgG |
| Clonality | Monoclonal |
| Immunogen | SARS-CoV-2 nucleoprotein (NP) specific peptide |
| Formulation | 1X PBS, 0.02% NaN3 |
| Isotype | Rabbit IgGk |
| Preparation | Protein A |
| Recommended Usage | Used for specific, high-sensitivity detection of SARS-CoV-2 nucleocapsid protein (NP) in immunoassay. Can be paired with other NP-specific clones for detection in sandwich ELISA format. |
| Storage | -20ºC |
| Pseudonyms | Severe acute respiratory syndrome coronavirus 2 nucleoprotein, 2019-nCoV nucleocapsid protein |
| Uniprot ID | P0DTC9 |
