Phospho-HS1 (Tyr397) (F12) rabbit mAb FITC Conjugate
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2398
HS1 is expressed in lymphoid and hematopoietic cells, and is heavily post-translationally modified. HS1 deficient mouse models have demonstrated the protein's role in receptor-mediated apoptosis and proliferation. HS1 is phosphorylated at Tyr378 and Tyr397 by the kinase Syk, providing a high-affinity binding site for SH2 domains from the Src family. Following this interaction, HS1 is then phosphorylated at Tyr222 by c-Fgr, Lyn, and Fyn kinases. HS1 plays an important role in T cell signaling, where HS1 phosphorylation recruits and activates Vav1 at the immune synapse. As a homolog of the actin binding protein cortactin, HS1 has been shown to mediate neutrophil chemotaxis through phosphorylation of tyrosines 222, 378, and 397.
| Applications | Flow Cytometry |
|---|---|
| Clone | HS1Y397-F12 |
| Format | FITC |
| Validated Reactivity | Human, Mouse |
| Cross Reactivity | Predicted to work with mouse, rat and other homologues. |
| Clonality | Monoclonal |
| Immunogen | A synthetic phospho-peptide corresponding to residues surrounding Tyr397 of human phospho HS1 |
| Formulation | 1X PBS, 0.09% NaN3, 0.2% BSA |
| Isotype | Rabbit IgGk |
| Preparation | Protein A+G |
| Recommended Usage | For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells or 5 µL per 100 µL of staining volume. It is recommended that the reagent be titrated for optimal performance for each application. See product image legends for additional information. |
| Storage | 2-8ºC |
| Pseudonyms | Hematopoietic lineage cell-specific protein, Hematopoietic cell-specific LYN substrate 1, LckBP1, p75, HCLS1 |
| Uniprot ID | P14317 |
| References | Brunati AM, et al., (1999) Journal of Biological Chemistry. 274:7557-7564. Cavnar PJ, et al., (2012) Journal of Biological Chemistry. 287: 25466-25477. |
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