Phospho-Zap70 (Tyr493)/Syk (Tyr526) (H11) rabbit mAb APC conjugate
ZAP70 (Tyrosine-protein kinase ZAP-70, phospho Zap70) is a protein tyrosine kinase (PTK) that associates with the z subunit of the T cell antigen receptor (TCR) and undergoes tyrosine phosphorylation following TCR stimulation. Following TCR engagement, Zap-70 is rapidly phosphorylated on several tyrosine residues through autophosphorylation and transphosphorylation by the Src family tyrosine kinase Lck. ZAP70 contains two SH2-like domains with the PTK domain located at the C-terminus. It appears that both phospho Zap70 and Syk are recruited to the phosphorylated CD3 and z subunits after TCR stimulation. Phosphorylation of Tyr319 is required for the assembly of a phospho Zap70-containing signaling complex that leads to the activation of the PLC-gamma1-dependent and Ras-dependent signaling cascades in antigen-stimulated T cells. The orthologous Tyr352 residue in Syk is also involved in the association with PLC-gamma1.
| Applications | Flow Cytometry |
|---|---|
| Clone | Zap70Y493-H11 |
| Format | APC |
| Validated Reactivity | Human, Mouse |
| Cross Reactivity | Predicted to work with mouse, rat and other homologues. |
| Clonality | Monoclonal |
| Immunogen | A synthetic phospho-peptide corresponding to residues surrounding Tyr493 of human phospho Zap70 |
| Formulation | 1X PBS, 0.09% NaN3, 0.2% BSA |
| Isotype | Rabbit IgGk |
| Preparation | Protein A+G |
| Recommended Usage | For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells or 5 µL per 100 µL of staining volume. It is recommended that the reagent be titrated for optimal performance for each application. |
| Storage | 2-8ºC |
| Pseudonyms | Tyrosine-protein kinase ZAP-70 , 70 kDa zeta-chain associated protein, Syk-related tyrosine kinase, SRK, Tyrosine-protein kinase SYK, Spleen tyrosine kinase, p72-Syk |
| Uniprot ID | P43403 P43405 |
| References | 1. Brdicka T et al., (2005) Mol Cell Biol 25:4924–4933. 2. Chan AC et al., (1992) Cell 71:649–662. 3. Cheng AM et al., (1997) Proc Natl Acad Sci94:9797–9801. 4. Deindl S et al., (2007) Cell 129:735–746. 5. Elder ME et al., (1994) Science 264:1596–1599. 6. Negishi I et al., (1995) Nature 376:435–438. 7. Yokosuka T et al., (2005) Nat Immunol 6:1253–1262. |
