Phospho-Lck (Tyr505) (A3) rabbit mAb PE conjugate
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Lck is a member of the Src family of non-receptor tyrosine kinases and plays a major role in T cell activation. Lck activates many downstream signaling pathways including Akt/mTOR, SAPK/JNK, PLCγ1, and RAS/MAPK. Phosphorylation of Lck at Tyr394 in the catalytic domain at the ATP-binding site stabilizes the open and active form, while phosphorylation at Tyr505 in the C-terminal domain promotes the closed, inactive conformation. Multiple small-molecule drugs used to treat leukemia have been shown to target inhibition of Lck, including imatinib and dasatinib. Lck is thus a promising target for suppressing T-cell responses for the treatment of inflammatory diseases or after organ transplantation.
| Applications | Flow Cytometry |
|---|---|
| Clone | LckY505-A3 |
| Format | PE |
| Validated Reactivity | Human |
| Cross Reactivity | Predicted to work with mouse, rat and other homologues. |
| Clonality | Monoclonal |
| Immunogen | A synthetic phospho-peptide corresponding to residues surrounding Tyr505 of human phospho Lck |
| Formulation | 1X PBS, 0.09% NaN3, 0.2% BSA |
| Isotype | Rabbit IgGk |
| Preparation | Protein A+G |
| Recommended Usage | For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells or 5 µL per 100 µL of staining volume. It is recommended that the reagent be titrated for optimal performance for each application. |
| Storage | 2-8ºC |
| Pseudonyms | Tyrosine-protein kinase Lck, Leukocyte C-terminal Src kinase, LSK, Protein YT16, T cell-specific protein-tyrosine kinase |
| Uniprot ID | P06239 |
| References | Serafin V, Capuzzo G, Milani G, et al. (2017) Blood. 130: 2750-2761. Lee KC, Ouwehand I, Giannini AL, et al. (2010) Leukemia. 24: 896-900. |
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