Recommended Pair - SARS-CoV-2 Nucleoprotein (NP) (75G5a) rabbit mAb

For highest sensitivity, we recommend pairing unconjugated clone 75G5a (Abwiz Cat. #2481), immobilized on the ELISA plate to capture COVID-19 NP antigen, with biotin-conjugated clone 85C10 (Abwiz Cat. #2507) for detection using high-sensitivity streptavidin-HRP (Pierce #21130). Other unconjugated and biotinylated anti-NP clones are provided as alternatives and also show high sensitivity and specificity.

We have leveraged our next-generation rabbit mAb discovery platform to develop recombinant rabbit monoclonal antibodies with extremely high sensitivity and specificity to SARS-CoV-2. These antibodies, which include clones 75G5a (Abwiz Cat. #2481), 84C4a (Abwiz Cat. #2486), 84D7 (Abwiz Cat. #2491), and 85C1 (Abwiz Cat. #2496) can be paired in sandwich detection assay and used to detect nucleoprotein (NP) antigen from SARS-CoV-2. Antibodies 75G5a, 84C4a, and 84D7 do not cross-react to the highly related SARS-CoV virus or to any other coronaviruses tested. Sandwich ELISA detection using TMB/acid developer reliably detects NP antigen in the pg/mL range, and sensitivity is expected to be even higher when using more sensitive developer strategies. This panel of antibodies can be used as raw materials for diagnostic kits and can be applied to lateral flow systems for diagnostic detection of COVID-19.